Coding

Part:BBa_K4650001:Design

Designed by: HuiChing Kuo   Group: iGEM23_KCIS-Xiugang-Taipei   (2023-07-21)


cellulose binding domain (CBD)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 499
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 499
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 51


Design Notes

REFERENCES 1. (Park and ROI,2000).


Source

Several research studies showed that the CBDs in the fungus and bacteria have high affinity for crystalline forms of cellulose. The researchers also determined that CBDs in fungus are normally smaller than CBDs in bacteria so our team has decided to use CBD protein in Schizosaccharomyces pombe (S. pombe)

References